|
KMID : 0895820200300040001
|
|
Journal of Oriental Rehabilitation Medicine
2020 Volume.30 No. 4 p.1 ~ p.16
|
|
|
Effect of Pahyeolsandong-tang (Poxiesanteng-tang) in Tibia Fracture-induced Mice
|
|
Shin Woo-Suk
Parichuk Kira
Cha Yun-Yeop
|
|
|
Abstract
|
|
|
Objectives: The main purpose of this study was to evaluate the bone healing effect of Pahyeolsandong-tang (PHT)(Poxiesanteng-tang) extract in tibia fracture-induced mice.
Methods: PHT was extracted using a solution of 35% ethanol in 60°C for 8 hours. Mice were randomly divided into 4 groups (normal, control, PHT 50 and PHT 100). Mice of experimental groups were medicated with PHT 50 or 100 mg/kg for 7 to 21 days. To clarify the effect of bone fracture healing, relative messenger RNA (mRNA) expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), osterix (OSX), Sox9, collagen type Ⅱ alpha 1 chain (Col2a1), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) were examined.
Results: In in vitro experiment, relative mRNA expression of OCN, Runx2, Col2a1 was significantly increased in PHT treated group to compare with control differentiation group. In in vivo experiment, relative mRNA expression of OCN, Runx2, OSX, Sox9, Col2a1, RANKL, OPG was significantly increased in PHT treated group.
Conclusions: This study showed that PHT accelerates bone fracture healing through the activation of osteoclasts and osteoblasts. It was showed that PHT significantly promotes osteoblasts differentiation by osteoblast differentiation markers such as OCN, Runx2, Col1a2. Also it was investigated that PHT had stimulatory effect on osteoblasts function through enhancing OCN, Runx2, OSX, Sox9, Col2a1 and, osteoclasts function through enhancing RANKL and OPG markers. PHT effectively promotes bone fracture healing process through activation of osteoblasts and osteoclasts
|
|
|
KEYWORD
|
|
|
Pahyeolsandong-tang (Poxiesanteng-tang), Osteoblasts, Osteoclasts, Chondrogenesis, Fracture healing
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|